Colorado, JulianJulianColoradoGómez, MauricioMauricioGómezGuacabare, DirceuDirceuGuacabareLópez, WilliamWilliamLópezMartínez, PaolaPaolaMartínezTorres, CésarCésarTorresAvellaneda, PatriciaCaycedo, Martha Lucia2026-10-022026-10-022006https://repositorio.unicoc.edu.co/handle/SII-Unicoc/3126Purpose: To observe the antibacterial activity of calcium hydroxide combined with various vehicles—anesthetic without epinephrine, anesthetic with epinephrine, glycerin, distilled water, saline solution, and chlorhexidine—against *Enterococcus faecalis*. Materials and Methods: *Enterococcus faecalis* samples were obtained from the National Institute of Health and cultured in sterile trypticase broth, with growth verified after 24 hours. Three Petri dishes containing Mueller-Hinton agar were used; the McFarland scale method was employed to standardize the homogeneous inoculation of *Enterococcus faecalis* in each dish. Subsequently, calcium hydroxide was mixed with the following vehicles: Group 1: anesthetic without epinephrine; Group 2: anesthetic with epinephrine; Group 3: glycerin; Group 4: distilled water; Group 5: saline solution; Group 6: chlorhexidine. Six paper discs were impregnated with the mixtures and placed in each dish. Data were recorded on a chart, categorizing sensitivity as: absent, mild, moderate, or marked. Results: After 24 hours, an absence of sensitivity was observed in Groups 1 and 4; mild sensitivity in Group 2; moderate sensitivity in Groups 3 and 6; and marked sensitivity in Group 5. Conclusions: Calcium hydroxide combined with anesthetic without epinephrine or distilled water showed no sensitivity. Moderate sensitivity was observed with calcium hydroxide combined with glycerin, chlorhexidine, and anesthetic with epinephrine. Calcium hydroxide combined with saline solution showed marked sensitivity against *Enterococcus faecalis*.Propósito: Observar la actividad antibacterial del Hidróxido de Calcio asociado a los vehículos Anestesia sin epinefrina, Anestesia con epinefrina, Glicerina, Agua Destilada, Suero Fisiológico, Clorhexidina sobre el Enterococo Faecalis. Materiales y Método: El Enterococo Faecalis se obtuvo en el Instituto Nacional de Salud, se cultivó en caldo estéril tripticasa, verificando a las 24 horas su crecimiento. Se tomaron 3 cajas Petri con agar Muller Hilton, se hizo la prueba de Escala de Mackfaller estandarizando así la siembra homogénea del Enterococo Faecalis en cada una de las cajas. Seguidamente se mezcló el hidróxido de calcio así: Grupo 1: anestesia sin epinefrina; Grupo 2: Anestesia con epinefrina; Grupo 3: Glicerina; Grupo 4: Agua destilada; Grupo 5: Suero fisiológico; Grupo 6: Clorhexidina. Se impregnaron y se colocaron en cada caja 6 discos de papel. Los datos se recolectaron en una ficha categorizando la sensibilidad así: ausente, leve, moderada, marcada. Resultados: a las 24 horas se observó ausencia de sensibilidad en los grupos 1 y 4; leve grupo 2; moderada grupos 3 y 6; y marcada grupo 5. Conclusiones: El hidróxido de calcio asociado con anestesia sin epinefrina y con agua destilada no mostró leve sensibilidad. Hubo moderada sensibilidad del hidróxido de calcio asociado con glicerina, clorhexidina y anestesia con epinefrina. El hidróxido de calcio asociado al suero fisiológico mostró marcada sensibilidad al enterococo faecalis.33 pp.application/pdfesEnterococo FaecalisBactericidaHidroxido de CalcioMedios de cultivoEstudios primariostext::thesis::bachelor thesisTO 1102Enterococcus faecalisBactericideCalcium hydroxideCulture mediaopenAccess