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Título
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Effect of Alendronate on Healing of Bone Defect and Gingival Tissue in Osteopenic Rats
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Description
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El objetivo fue evaluar la reparación ósea y gingival en ratas con osteopenia. Quince ratas wistar hembras fueron incluidas; en todas ellas se realizo ovarectomia y fue realizada la inducción de osteopenia; después de 45 días, los animales fueron sometidos a dos técnicas quirúrgicas 1) extracciones dentales del incisivo central superior sin preservación alveolar y 2) creación de un defecto craneano de 5 mm en la calota; 5 animales fueron incluidos como grupo control (G1) sin la aplicación de alendronato; en el grupo 2 (G2) se utilizó alendronato subcutáneo (0,5 mg/kg) una vez a la semana durante 3 semanas. En el grupo 3 (G3), después de la ovarectomia se realizó la exodoncia y el defecto en el cráneo y después de ello se inicio el protocolo con alendronato. En cada grupo, después de seis semanas se realizó la eutanasia con descripción histológica de los hallazgos. En el hueso formado en el defecto craneano de 5 mm se observó una adecuada progresión de reparación en los 3 modelos experimentales y no se observó cambios importantes en el modelo de reparación. Para el tejido gingival en el sitio de extracción, no se observaron diferencias entre el grupo G1 y G3. Por otra parte, el G2 presentó un tejido mas delgado con reducción del epitelio gingival; nuestros resultados demuestran que el alendronato no fue un obstáculo en la reparación ósea; deficiencias en la re epitelización de la mucosa oral muestran el impacto del alendronato después de la exodoncia.
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Relation
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Grupo de Ciencias Odontológicas Unicoc
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Creator
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Sigua Rodriguez, Eder Alberto
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Santana Quinto, José Henrique
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Marçal Natali, Maria Raquel
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Olate, Sergio
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Nakamura Cuman, Roberto Kenji
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Jacobucci Farah, Gustavo
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Mediator
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Centro de Investigación Colegio Odontológico - CICO
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Date Issued
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2020
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Abstract
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The aim was to evaluate bone repair and gingival tissue repair in osteopenic rats. Fifteen female wistar rats were included; in all of them ovariectomy was realized to induce osteopenia; after 45 days, the animals were submitted to 2 surgical techinques 1) dental extraction of the upper central incisor with no socket preservation and 2) 5 mm cranial defect in the calvarium; 5 rats were included in the control group (G1) withput alendronate application; in the group 2 (G2) was used subcutenous alendronate (0.5 mg/kg) once for three weeks and then was realizd the both surgical techniques. In group 3 (G3), after ovariectomy was realized the both dental extraction and the calvarium defect and after that was realized the alendronate protocol. In each group, after six week was realized euthanasia and descriptive histological analysis of the surgical areas involved. In bone formation of the 5 mm cranial defect was observed with good progression in the 3 experimental models and no modification in quality of bone repair was observed. For the gingival tissue in the extraction socket, no differences were observed between G1 and G3. On other hand, in G2 a thinner and reduced gingival epithelium was found. Our results showed that alendronate was not an obstacle for bone repair; deficiencies in re-epithelialization of oral mucosa show the impact of alendronate before dental extraction.
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Extent
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6 pp.
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Format
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application/pdf
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issn
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0717-9502
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Lenguaje
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Inglés
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Publisher
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Sociedad Chilena de Anatomía
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page end
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688
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issue
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3
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page start
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683
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Fuente
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International Journal of Morphology
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Int. J. Morphol
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Repositorio Institucional Unicoc - RI-Unicoc
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volume
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38
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Is Part Of
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International Journal of Morphology, ISSN: 0717-9502, Vol. 38, No. 3, (2020), pp. 683-688
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Access Rights
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openAccess
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Bibliographic Citation
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Agis, H.; Blei, J.; Watzek, G. & Gruber, R. Is zoledronate toxic to human periodontal fibroblasts? J. Dent. Res., 89(1):40-5, 2010.
-
Brozoski, M. A.; Traina, A. A.; Deboni, M. C.; Marques, M. M. & NaclérioHomem, M. da G. Bisphosphonate-related osteonecrosis of the jaw. Rev. Bras. Reumatol., 52(2):265-70, 2012.
-
Canettieri, A. C. V.; Colombo, C. E. D.; Chin, C. M. & Faig-Leite, H. Femur bone repair in ovariectomized rats under the local action of alendronate, hydroxyapatite and the association of alendronate and hydroxyapatite. Int. J. Exp. Pathol., 90(5):520-6, 2009.
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Cooper, G. M.; Mooney, M. P.; Gosain, A. K.; Campbell, P. G.; Losee, J. E. & Huard, J. Testing the critical size in calvarial bone defects: revisiting the concept of a critical-size defect. Plast. Reconstr. Surg., 125(6):1685- 92, 2010.
-
Dimitrakopoulos, I.; Magopoulos, C. & Karakasis, D. Bisphosphonateinduced avascular osteonecrosis of the jaws: a clinical report of 11 cases. Int. J. Oral Maxillofac. Surg., 35(7):588-93, 2006.
-
Kumar, V. & Sinha, R. K. Evolution and etiopathogenesis of bisphosphonates induced osteonecrosis of the jaw. N. Am. J. Med. Sci., 5(4):260-5, 2013.
-
Landesberg, R.; Cozin, M.; Cremers, S.; Woo, V.; Kousteni, S.; Sinha, S.; Garrett-Sinha, L. & Raghavan, S. Inhibition of oral mucosal cell wound healing by bisphosphonates. J. Oral Maxillofac. Surg., 66(5):839-47, 2008.
-
Li, M.; Shen, Y. & Wronski, T. J. Time course of femoral neck osteopenia in ovariectomized rats. Bone, 20(1):55-61, 1997.
-
Lin, J. T. & Lane, J. M. Osteoporosis: a review. Clin. Orthop. Relat. Res., (425):126-34, 2004.
-
McLeod, N. M.; Moutasim, K. A.; Brennan, P. A.; Thomas, G. & Jenei, V. In vitro effect of bisphosphonates on oral keratinocytes and fibroblasts. J. Oral Maxillofac. Surg., 72(3):503-9, 2014.
-
Migliorati, C. A.; Schubert, M. M.; Peterson, D. E. & Seneda, L. M. Bisphosphonate-associated osteonecrosis of mandibular and maxillary bone: an emerging oral complication of supportive cancer therapy. Cancer, 104(1):83-93, 2005.
-
Mozzati, M.; Arata, V. & Gallesio, G. Tooth extraction in osteoporotic patients taking oral bisphosphonates. Osteoporos. Int., 24(5):1707-12, 2013.
-
Ohashi, Y.; Aihara, E.; Takasuka, H.; Takahashi, K. & Takeuchi, K. Antral ulcers induced by alendronate, a nitrogen-containing biphophonate, in rat stomachs - prophylactic effect of rebamipide. J. Physiol. Pharmacol., 60(3):85-93, 2009.
-
Oliveira, D.; Hassumi, J. S.; Gomes-Ferreira, P. H.; Polo, T. O.; Ferreira, G. R.; Faverani, L. P. & Okamoto, R. Short term sodium alendronate administration improves the peri-implant bone quality in osteoporotic animals. J. Appl. Oral Sci., 25(1):42-52, 2017.
-
Paiva-Fonseca, F.; Santos-Silva, A. R.; Della-Coletta, R.; Vargas, P. A. & Lopes, M. A. Alendronate-associated osteonecrosis of the jaws: A review of the main topics. Med. Oral Patol. Oral Cir. Bucal, 19(2):e106-11, 2014.
-
Papapetrou, P. D. Bisphosphonate-associated adverse events. Hormones (Athens), 8(2):96-110, 2009.
-
Ravosa, M. J.; Ning, J.; Liu, Y. & Stack, M. S. Bisphosphonate effects on the behaviour of oral epithelial cells and oral fibroblasts. Arch. Oral Biol., 56(5):491-8, 2011.
-
Reid, I. R.; Bolland, M. J. & Grey, A. B. Is bisphosphonate-associated osteonecrosis of the jaw caused by soft tissue toxicity? Bone, 41(3):318- 20, 2007.
-
Ruggiero, S. L. & Woo, S. B. Biophosphonate-related osteonecrosis of the jaws. Dent. Clin. North Am., 52(1):111-28, 2008.
-
Ruggiero, S. L.; Dodson, T. B.; Fantasia, J.; Goodday, R.; Aghaloo, T.; Mehrotra, B.; O’Ryan, F. & American Association of Oral and Maxillofacial Surgeons. American Association of Oral and Maxillofacial Surgeons position paper on medication-related osteonecrosis of the jaw- -2014 update. J. Oral Maxillofac. Surg., 72(10):1938-56, 2014.
-
Scheper, M. A.; Badros, A.; Chaisuparat, R.; Cullen, K. J. & Meiller, T. F. Effect of zoledronic acid on oral fibroblasts and epithelial cells: a potential mechanism of bisphosphonate-associated osteonecrosis. Br. J. Haematol., 144(5):667-76, 2009.
-
Yano, T.; Yamada, M.; Konda, T.; Shiozaki, M. & Inoue, D. Risedronate improves bone architecture and strength faster than alendronate in ovariectomized rats on a low-calcium diet. J. Bone Miner. Metab., 32(6):653-9, 2014.
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primary topic
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Research Subject Categories::ODONTOLOGY::Cell and molecular biology
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Subject
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Biphosphonate
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Osteonecrosis
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Osteoporosis
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Bifosfonato
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Osteoporosis
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Osteonecrosis
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Alternative Title
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Efecto del Alendronato en la Reparación de Defectos Óseos y Tejido Gingival en Ratas con Osteopenia
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Tipo
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Artículo
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current project
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Grupo de Ciencias Odontológicas Unicoc
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number of pages
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6 pp.
